Small Molecule Compound Cryo-Electron Microscopy (Micro ED Technology) Structure Analysis

        Micro ED Structure Analysis Technology: Microcrystal Electron Diffraction Technology (Micro ED Technology) is a technique for determining the three-dimensional spatial structure of crystals based on electron diffraction data obtained by cryo-transmission electron microscopy.
        This technology uses high-energy electrons instead of traditional X-rays as the radiation source, achieving a 10-fold increase in structure analysis efficiency and doubling the success rate. Therefore, only a small amount of micro/nano-sized (>0.1um) crystal grains are needed to quickly and efficiently obtain high-resolution diffraction data, significantly reducing requirements for sample shape, purity, and size.
        Traditional crystal structure analysis methods use X-rays to diffract sample crystals, then collect diffraction data, and then restore the diffraction data to crystal structure through algorithms. X-rays have strong penetration ability but interact weakly with matter, so sufficiently large samples are required to generate enough signal. Micro ED technology uses high-energy electrons instead of X-rays, utilizing the strong interaction between electrons and matter, and only nano-sized crystals are needed to generate sufficient signal. Microcrystal electron diffraction technology reduces the characterization threshold by 108 times, allowing rapid acquisition of small molecule structures, thus significantly shortening the structure confirmation cycle.
        Currently, Micro ED structure analysis tests have been conducted on over 150 small molecule compounds, forming preliminary unique solutions for cryo-sample preparation, electron microscopy scanning, and data analysis, which can be provided as external services. We welcome colleagues engaged in innovative technology research of natural products and small molecule compounds for guidance and cooperation.